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Image Search Results
Journal: Nature Communications
Article Title: Intracellular hydrogelation preserves fluid and functional cell membrane interfaces for biological interactions
doi: 10.1038/s41467-019-09049-5
Figure Lengend Snippet: Examination of membrane protein lateral mobility on GCs. a Representative trajectories from CD80-GFP fluorescence tracking on different GCs and control cells examined by TIRF microscopy. b Diffusion coefficients of CD80-GFP were calculated from the TIRF fluorescence tracking data. Error bars represent geometric mean with 95% confidence interval. c Representative images showing recovery of CD80-GFP fluorescence in 4 wt% GC following photobleaching. Red rectangles indicate the photobleached area of interest. Scale bars = 2 μm. d A schematic illustration of membrane proteins with different sizes of intracellular domains, including CD80-GFP, EGFP-GPI, TfR, Lyn-GFP, and EGFR-GFP, which were assessed for their lateral mobility on GCs. e Recovery kinetics of CD80-GFP, EGFP-GPI, TfR, Lyn-GFP and EGFR-GFP on GCs and control cells assessed by FRAP. For GCs with 4 to 20 wt% hydrogel densities, all examined membrane proteins had similar lateral mobility as on live cells. Error bars represent geometric mean with 95% confidence interval. f Membrane protein mobility on 40 wt% GCs relative to their corresponding mean mobility on live cells. Error bars represent geometric mean with 95% confidence interval ( n = 7–12). Statistical analysis was performed using a two-tail Student t test, **** p <0.001
Article Snippet: HeLa cells expressing EGFP-GPI (Addgene, pCAG: GPI-GFP, #32601), CD80-GFP (Sino Biological Inc., pCMV3-mCD80-C-GFPSpark, MG50446-ACG), Transferrin Receptor (TfR) (Sino Biological Inc., pCMV3-hTfR-C-DDK (flag) tag, HG11020-CF), Lyn-GFP (Sino Biological Inc., pCMV3-hLyn-C-GFPSpark, HG10829-ACG),
Techniques: Fluorescence, Microscopy, Diffusion-based Assay